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pbluescript ii ks+ cloning vector, apr  (Agilent technologies)


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    Agilent technologies pbluescript ii ks+ cloning vector, apr
    Pbluescript Ii Ks+ Cloning Vector, Apr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks++cloning+vector%2C+apr/pm20630728-46-58-59
    Average 90 stars, based on 1 article reviews
    pbluescript ii ks+ cloning vector, apr - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Identification and Characterization of Genes Required for Biosynthesis and Transport of the Siderophore Vibrioferrin in Vibrio parahaemolyticus
    Article Snippet: Strains and plasmids used in this study Strain or plasmid Description Reference or source Strains V. parahaemolyticus WP1 Clinical isolate 17 AQ3354 Clinical isolate 17 TNB1 AQ3354, pvsA disrupted; Cmr This study TNB2 AQ3354, pvsD disrupted; Cmr This study TNB3 AQ3354, pvsE disrupted; Cmr This study TNB4 AQ3354, pvuB disrupted; Cmr This study E. coli DH5 endA1 hsdr17 (rK mK ) supE44 thi-1 recA1 gyrA96 relA1 (argF- lacZYA)U169 deoR [ 80d lac (lacZ)M15)]; general cloning host Promega JM109 recA1 endA1 gylA96 thi hsdR17 supE44 relA1 (lac-proAB)/F (traD36 proAB lacIq lacZ M15); general cloning host 72 SY327 pir (lac pro) argE(Am) recA56 gyrA rpoB pir, host for -requiring plasmids 35 SM10 pir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu, pir; Kmr; host for - requiring plasmids; conjugal donor 35 Plasmids pUC19 High-copy-number cloning vector; Apr 61 pBluescript II KS( ) High-copy-number cloning vector; Apr Stratagene pMW118 Low-copy-number cloning vector; Apr Nippon Gene pACYC184 Low-copy-number cloning vector; Tcr Cmr 8 pKTN701 R6K-ori suicide vector for gene replacement; Cmr 39 pVP3151 Initially isolated FURTA-positive clone; pUC19 containing chromosomal 3,151-bp PstI fragment from WP1; Apr 17 pVS1 pMW118 containing chromosomal 3.2-kb SalI-SalI fragment from WP1; Apr This study pVS1-1 pBluescript II KS( ) containing 373-bp PstI-KpnI fragment from pVS1; Apr This study pVS2 pMW118 containing chromosomal 7.5-kb HindIII-HindIII fragment from WP1; Apr This study pVPV2995 pBluescript II KS( ) containing the chromosomal 2,995-bp EcoRI-SacI fragment from WP1; Apr 17 pVFRD1 pACYC184 containing chromosomal 7.7-kb SalI-HindIII fragment from WP1; Cmr This study pTNB1 pKTN701 containing 682-bp XbaI-EcoRI fragment within pvsA from pVS2; Cmr This study pTNB2 pKTN701 containing 748-bp SacI-KpnI fragment PCR amplified with primers PVSD-Kpn and PVSD-Sac with pVS2 as a template; Cmr This study pTNB3 pKTN701 containing 688-bp KpnI-EcoRI fragment PCR amplified with primers PVSE-Kpn and PVSE-Eco with pVS2 as a template; Cmr This study pTNB4 pKTN701 containing 601-bp SacI-KpnI fragment PCR amplified with primers PVUB-Sac and PVUB-Kpn with pVFRD1 as a template; Cmr This study on M arch 18, 2015 by U C LA B IO M E D IC A L LIB /S E R IA LS http://jb.asm .org/ D ow nloaded from membrane for colony and plaque hybridization (Roche Diagnostics) were denatured and neutralized, and the transferred DNA was fixed to the membrane by baking it for 30 min at 120°C.

    Cloning:

    Article Title: Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
    Article Snippet: .. KW251 supE44 supF58 hsdr514 galK2 galT22 metB1 trpR55 lacY1 recD mcrA mcrB, derivative of E. coli LE392 for propagation of phage vectors Promega JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi D(lac-proAB) F9(traD36 proAB22 lacIq lacZDM15) 48 XL-1 Blue supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F9(traD36 proAB1 lacIq lacZDM15) Stratagene Inc. C. testosteroni ATCC 17410 tes1 N. J. Palleroni P. putida KT2440 mt-2 r2m1, derivative of Pseudomonas arvilla S. Harayama Plasmids pUC19 Multipurpose cloning vector, Apr 48 pTES80 pUC19 derivative with a 7.8-kbp SalI DNA fragment from C. testosteroni ATCC 17410 containing D1dh, Apr 37 pBA160 pUC19 derivative with a 16-kbp BamHI DNA fragment from C. testosteroni ATCC 17410 containing D1- and D4dh, Apr 37 pTEK21 pUC derivative with a 2.2-kbp KpnI fragment from pTES80 containing D1dh, Apr 37 pXE25 pUC19 derivative with a 2.5-kbp XhoI-EcoRI fragment from pBA160 containing D4dh, Apr This study pRG1 pACYC177 derivative with the lacIq gene 14 pBlueScript II KS(1) Phagemid derivative of pUC19 containing f1 origin of replication which allows rescue of single-stranded DNA upon coinfection with helper phage, Apr Stratagene Inc. pBHE25 2.5-kbp HindIII-EcoRI fragment from pXE25 in pBlueScript II KS(1), Apr This study pBHN17 Derivative of pBHE25 with a 1.8-kbp NotI-EcoRI fragment deleted, Apr This study pBHS16 Derivative of pBHE25 with a 1.9-kbp SmaI-EcoRI fragment deleted, Apr This study pBKE22 Derivative of pBHE25 with a 0.3-kbp XhoI-KpnI fragment deleted, Apr This study pMMB208 Broad-host-range expression vector, Cmr 33 pMMB25 pMMB208 containing 2.5-kbp HindIII-EcoRI fragment from pXE25 carrying D4dh, Cmr This study pGP1-2 pACYC177 derivative providing for heat-sensitive expression of T7 RNA polymerase, Kmr 43 pT7-4 Plasmid with the T7 RNA promoter f10, Apr 43 pT7-HE25 2.5-kbp HindIII-EcoRI fragment from pXE25 in pT7-4, Apr This study pT7-HH13 1.3-kbp HindIII-HindIII fragment from pXE25 in pT7-4, Apr This study Phages lGem11 Multifunctional cloning vector Promega R408 Helper phage for pBluescript II KS(1) packaging Stratagene a Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin. ..

    Expressing:

    Article Title: Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
    Article Snippet: .. KW251 supE44 supF58 hsdr514 galK2 galT22 metB1 trpR55 lacY1 recD mcrA mcrB, derivative of E. coli LE392 for propagation of phage vectors Promega JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi D(lac-proAB) F9(traD36 proAB22 lacIq lacZDM15) 48 XL-1 Blue supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F9(traD36 proAB1 lacIq lacZDM15) Stratagene Inc. C. testosteroni ATCC 17410 tes1 N. J. Palleroni P. putida KT2440 mt-2 r2m1, derivative of Pseudomonas arvilla S. Harayama Plasmids pUC19 Multipurpose cloning vector, Apr 48 pTES80 pUC19 derivative with a 7.8-kbp SalI DNA fragment from C. testosteroni ATCC 17410 containing D1dh, Apr 37 pBA160 pUC19 derivative with a 16-kbp BamHI DNA fragment from C. testosteroni ATCC 17410 containing D1- and D4dh, Apr 37 pTEK21 pUC derivative with a 2.2-kbp KpnI fragment from pTES80 containing D1dh, Apr 37 pXE25 pUC19 derivative with a 2.5-kbp XhoI-EcoRI fragment from pBA160 containing D4dh, Apr This study pRG1 pACYC177 derivative with the lacIq gene 14 pBlueScript II KS(1) Phagemid derivative of pUC19 containing f1 origin of replication which allows rescue of single-stranded DNA upon coinfection with helper phage, Apr Stratagene Inc. pBHE25 2.5-kbp HindIII-EcoRI fragment from pXE25 in pBlueScript II KS(1), Apr This study pBHN17 Derivative of pBHE25 with a 1.8-kbp NotI-EcoRI fragment deleted, Apr This study pBHS16 Derivative of pBHE25 with a 1.9-kbp SmaI-EcoRI fragment deleted, Apr This study pBKE22 Derivative of pBHE25 with a 0.3-kbp XhoI-KpnI fragment deleted, Apr This study pMMB208 Broad-host-range expression vector, Cmr 33 pMMB25 pMMB208 containing 2.5-kbp HindIII-EcoRI fragment from pXE25 carrying D4dh, Cmr This study pGP1-2 pACYC177 derivative providing for heat-sensitive expression of T7 RNA polymerase, Kmr 43 pT7-4 Plasmid with the T7 RNA promoter f10, Apr 43 pT7-HE25 2.5-kbp HindIII-EcoRI fragment from pXE25 in pT7-4, Apr This study pT7-HH13 1.3-kbp HindIII-HindIII fragment from pXE25 in pT7-4, Apr This study Phages lGem11 Multifunctional cloning vector Promega R408 Helper phage for pBluescript II KS(1) packaging Stratagene a Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin. ..

    Plasmid Preparation:

    Article Title: Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
    Article Snippet: .. KW251 supE44 supF58 hsdr514 galK2 galT22 metB1 trpR55 lacY1 recD mcrA mcrB, derivative of E. coli LE392 for propagation of phage vectors Promega JM109 recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi D(lac-proAB) F9(traD36 proAB22 lacIq lacZDM15) 48 XL-1 Blue supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F9(traD36 proAB1 lacIq lacZDM15) Stratagene Inc. C. testosteroni ATCC 17410 tes1 N. J. Palleroni P. putida KT2440 mt-2 r2m1, derivative of Pseudomonas arvilla S. Harayama Plasmids pUC19 Multipurpose cloning vector, Apr 48 pTES80 pUC19 derivative with a 7.8-kbp SalI DNA fragment from C. testosteroni ATCC 17410 containing D1dh, Apr 37 pBA160 pUC19 derivative with a 16-kbp BamHI DNA fragment from C. testosteroni ATCC 17410 containing D1- and D4dh, Apr 37 pTEK21 pUC derivative with a 2.2-kbp KpnI fragment from pTES80 containing D1dh, Apr 37 pXE25 pUC19 derivative with a 2.5-kbp XhoI-EcoRI fragment from pBA160 containing D4dh, Apr This study pRG1 pACYC177 derivative with the lacIq gene 14 pBlueScript II KS(1) Phagemid derivative of pUC19 containing f1 origin of replication which allows rescue of single-stranded DNA upon coinfection with helper phage, Apr Stratagene Inc. pBHE25 2.5-kbp HindIII-EcoRI fragment from pXE25 in pBlueScript II KS(1), Apr This study pBHN17 Derivative of pBHE25 with a 1.8-kbp NotI-EcoRI fragment deleted, Apr This study pBHS16 Derivative of pBHE25 with a 1.9-kbp SmaI-EcoRI fragment deleted, Apr This study pBKE22 Derivative of pBHE25 with a 0.3-kbp XhoI-KpnI fragment deleted, Apr This study pMMB208 Broad-host-range expression vector, Cmr 33 pMMB25 pMMB208 containing 2.5-kbp HindIII-EcoRI fragment from pXE25 carrying D4dh, Cmr This study pGP1-2 pACYC177 derivative providing for heat-sensitive expression of T7 RNA polymerase, Kmr 43 pT7-4 Plasmid with the T7 RNA promoter f10, Apr 43 pT7-HE25 2.5-kbp HindIII-EcoRI fragment from pXE25 in pT7-4, Apr This study pT7-HH13 1.3-kbp HindIII-HindIII fragment from pXE25 in pT7-4, Apr This study Phages lGem11 Multifunctional cloning vector Promega R408 Helper phage for pBluescript II KS(1) packaging Stratagene a Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin. ..



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